- Overview
- Tissue culture facility for human iPSC lines
- Aquaneering Zebrafish Housing facility
- Pipette Puller and Microforge Apparatus
- Amaxa Nucleofection Apparatus
- Leica SP8X Confocal Microscope
- Leica DM6000 inverted fluorescent microscope
- Leica M60 dissecting microscope with digital imaging
- Zeiss Axiovert 200 Fluorescent Microscope
- Image Analyses Systems
- Beckman Optima-E Ultracentifuge
- Molecular Devices Spetramax i3 Plate Reader System
- Bio-Rad Droplet QX200 Digital qPCR system and CFX384 real-time PCR system
- Protein Simple Wes System
- LightScanner from Idaho Technologies
In addition to standard equipment needed for modern molecular/cell biological and biochemical studies, laboratories within the Center have established a number of specialized facilities and advanced instrumentation to support and advance our research. We provide these pages for our colleagues’ use – we will update these pages as new facilities and instrumentation are acquired in the Center. As noted in full descriptions, costs are incurred for use of some equipment and user must cover their own supplies and reagents.
This is a dedicated tissue culture facility for maintenance and differentiation of human induced pluripotent stem cells (iPSC). Use requires training, IBC approval, and permission from Dr. Lizarraga for any use. Additional tissue culture facilities for both cell lines and primary rodent cells/tissues are available in the Twiss, Smith, and Lizarraga labs.
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This an IACUC approved, automated facility for housing and breeding zebrafish lines. The facility is a secured, environmentally controlled room within the Poulain lab. Use requires IACUC approval, training, and permission from Dr. Poulain for any use.
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Sutter P1000 instrument for ‘pulling’ glass micropipettes and microforge for polishing/modifying glass micropipette tips is in the Poulain lab. These are used for single cell injections. Use requires training, IBC approval, and permission from Poulain lab.
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Both Amaxa II and 4D instruments (Lonza) for electroporation of eukaryotic cell lines and primary cells are available in the Twiss, Smith, and Lizarraga labs. Use requires training, IBC approval (also IACUC approvals if primary cells) and permission of the respective faculty member.
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The SP8X is configured with: 405 nm, 545 nm, and tunable pulsed white light lasers; 2 gated HyD detectors, 3 fluorescent PMTs and DIC PMT; AOBS and AOTF; resonant scanning; motorized XY stage, galvanometer Z stage, and definite focus system; and, full enclosure for live cell imaging (CO2 and temperature control). Use requires training and permission from Twiss lab staff; please discuss with Twiss for costs to use the instrument (costs offset our service contract).
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The DM6000 epifluorescent microscope is on an inverted platform and equipped with Hamamatsu ORCA ER CCD, motorized XY stage for tile scanning, and filters for DAPI, FITC, Cy3, and Cy5. Use requires training and permission from Twiss lab.
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This dissection scope is housed within a laminar flow hood for surgical dissections of rodent neurons for culture. The scope is fitted with a color camera to assist with training in surgical approaches. Manual micromanipulator and injection system is available for viral work. Use requires training, IACUC approval (also potentially IBC approval), and permission from Twiss lab.
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This inverted fluorescent microscope is equipped with heated stage and microinjection system. The imaging package allows for deconvolution of image stacks. Use requires training and permission from Smith lab.
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Several software packages are available for analyses and manipulation of microscopic images. These include Volocity, ImageJ, and Neuromath. NeuroLucida 360 will be added in the near future. Please discuss potential needs with Center faculty. We are constantly upgrading our capabilities.
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Optima-E is a tabletop microformat ultracentrifuge. Both fixed angle and swinging bucket rotors are available. Use requires training and permission from Dr. Smith.
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The Spectramax i3 system provides fluorescence, luminescence, visible light absorbance, and fluorescence polarization absorption in a variable microplate format. Use requires training and permission from Twiss lab.
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Two instruments are available for quantitative PCR studies. The CFX384 is an LED-based real-time PCR instrument for both TaqMan (duplex) and SybrGreen detection. The QX200 is a digital (end point) read out that can do both duplexed TaqMan probes and SybrGreen detection – this instrument provides a substantial drop in detection limits over classic real-time PCR.
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Wes is a capillary-based protein electrophoresis and automated immunoassay system that replaces classic Western blots. The system provides full quantitation and allows decreased inputs for both protein lysates and antibodies. Use requires training and permission from Twiss lab.
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The LightScanner System performs high-throughput mutation discovery and gene scanning using DNA melting analysis. Use requires training and permission from Dr. Poulain.
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